- CFX MANAGER TARGET STABILITY DRIVERS
- CFX MANAGER TARGET STABILITY FULL
- CFX MANAGER TARGET STABILITY SOFTWARE
- CFX MANAGER TARGET STABILITY SERIES
The selection of an appropriate reference gene is crucial and stable. Gene expression analyses rely on the use of reference genes, which are internal controls for varied samples and different experimental settings. As most researchers focus on analyzing the discrepant changes in gene mRNA expression levels, relative quantification has become a widely applied method for amplifying and detecting gene expression.
The absolute quantification for a particular transcript gene copy number is calculated by the standard curve, and the relative quantification for a target gene may be measured for the relative expression compared with the reference gene. IntroductionĪt present, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is considered to be a sensitive, accurate, highly specific, and fast measurement of gene expression with absolute or relative quantification. This technique may be applied to promote the selection process of the optimal reference gene in other experiments. Our results indicated that none of these 15 candidate reference genes can be used as reference gene individually for relative quantitative fluorescence PCR analysis however, the combination of Grcc10 and Ppia, based on the process of calculating the higher value and lower difference values between groups, was the best choice as a reference gene for the lung tissue samples in KYDS mice infected with IAV. Four additional host innate immune system-related genes (TLR3, TLR4, TLR7, and RIG-I) were analyzed together with the two treatment datasets to confirm the selected reference genes. A value > 0.05 and the lowest absolute value of the difference indicated the optimal reference two-gene combination. In the present study, we explored a novel method using dual-gene combinations the difference in gene expression between the model and normal control groups was statistically analyzed by an independent-samples -test, and the difference in the mean value between the two groups was compared. The results were authenticated over extended experimental settings by a group of 10 samples.
NormFinder, BestKeeper, and GeNorm were used to assess the stability of reference genes. In the present study, 15 candidate reference genes (Actb, β2m, Gapdh, Gusb, Tuba, Grcc10, Eif4h, Rnf187, Nedd8, Ywhae, 18S rRNA, Rpl13, Ubc, Rpl32, and Ppia) were investigated in lung cells from KYDS mice infected with IAV H1N1. In order to precisely evaluate the relative expression of genes in the cells of mice suffering from Kidney Yang Deficiency Syndrome (KYDS) in response to influenza A virus (IAV) H1N1 using RT-qPCR, it is crucial to identify reliable reference genes.
CFX MANAGER TARGET STABILITY FULL
Please refer specification pages for full details.In reverse transcription-quantitative polymerase chain reaction (RT-qPCR) studies, endogenous reference genes are routinely used to normalize the expression of target gene studies. * Specifications and features vary by model, and all images are illustrative. *Visit the ASUS website at for the latest CPU QVL (Qualified Vendors List). Please refer to the specifications sheet for details. *Some models without the 6.5MB Intel® Management Engine firmware do not support Intel ® SBA. *Local administrator rights on the target machine
CFX MANAGER TARGET STABILITY SOFTWARE
The Intel® Management Engine software kit must be installed (the Local Manageability Service and the Intel ® Management Engine Interface must be installed and running.) Intel® Core™ i3/i5/i7 with one of these chipsets: B150, H170, Q170
CFX MANAGER TARGET STABILITY SERIES
PCH with 6th-generation Intel® Core™ processor (100 Series platforms) with 6.5MB Intel® Management Engine (ME) 11.0 firmware installed
CFX MANAGER TARGET STABILITY DRIVERS
Intel® SBA requires MEI drivers (AMT host software kit) be installed. Intel® SBA (Small Business Advantage) is a combination of hardware and software that provides unique security and productivity capabilities designed for small businesses.